PEPperMAP® Affinity Determination Service

Analysis of peptide binding to your target protein/antibody by fluorescence polarization
Determination of K_D/EC₅₀ values under equilibrium conditions
Including a HPLC-purified peptide with fluorescein label
Reporting with assay protocol, dose-response curves and estimated K_D or EC₅₀ values

€1,795.00

Excl. 19% VAT

In stock

SKU

PPM.020.001

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Details

The PEPperMAP^® Affinity Determination Service enables the confirmation and affinity determination of peptides to a target protein or antigen in a soluble, homogeneous format using fluorescence polarization as a quantitative measure for protein-peptide binding.

The K_D/EC₅₀ values are determined by monitoring the binding of the target protein or antibody to a fixed amount of a labeled peptide with increasing protein concentrations. The fluorescence polarization is measured under equilibrium conditions followed by generation of a dose-response curve.

The comprehensive service covers the following items:

HPLC-purified peptide with fluorescein label: 0.5 mg of an HPLC-purified and fluorescein-labeled peptide is provided by PEPperPRINT.
Target protein or antibody: To be supplied by the customer.The dose-response curve is generated at concentrations between 0.001 μM and 1 μM and requires approximately 0.5 mg protein. Weaker ligands (K_D > 1 μM) are analyzed at higher protein concentrations; for measurements of up to 10 μM, 1 to 2 mg target protein is required, depending on the protein's molecular weight.
Fluorescence polarization measurements: The K_D/EC₅₀ values are determined by monitoring the binding of the target protein or antibody to a fixed amount of the labeled peptide with increasing protein concentrations. The fluorescence polarization is measured under equilibrium conditions followed by generation of a dose-response curve.
Report: The report comprises a description of the assay protocol, the dose-response curve and the estimated K_D or EC₅₀ value of the peptide ligand against the protein or antibody target.